What we offer
IRF staff support the provision of a histology suite that allows the histological and immunohistological analysis of cells and tissues. The facility currently provides the following:
Technique | Equipment |
Tissue Sectioning - Cryostat (frozen)
|
Leica CM1900 + Thermo Scientific HM525NX
|
Tissue Sectioning - Paraffin Wax
|
Leica RM2255 - Rotary Microtome
|
Tissue Embedding – Wax
|
Leica Histocore Arcadia H Embedding Station
Leica Histocore Arcadia C Cold Plate
Leica EGF Heatable Forceps
Leica Surgipath TrimEase Block Trimmer
|
Tissue Embedding - Cryostat
|
Hand processing
|
APES coated slides
|
Hand processing
|
Immunohistochemistry (IHC)
|
Hand processing
|
Histology Stain - Hematoxylin and Eosin
|
Hand processing
|
Histology Stain - Lillie's Trichrome
|
Hand processing
|
Service
Upon request technical staff can undertake work in the area of histology and immunohistochemistry on behalf of the facility users. Contact the facility for further information and pricing.
Requirements
Section specifications
If you are providing sections already mounted for histology or immunohistochemistry work they must be of an appropriate standard please read here for further information (Word).
Tissue specification
Discuss with Histology section technician. Further information upon enquiry.
DIY
Facility users can access the laboratories and perform techniques using our resources. The price is lower cost than service provision by facility staff. Contact the facility for further information and pricing.
Users can make use of our fully equipped laboratory and reagents for performing histology and immunohistochemistry staining. We can provide some primary antibodies upon request and at a cost. Secondary antibodies and immunohistochemistry Haematoxylin counterstain are inclusive in the price.
Requirements
Training
All users will need to be trained by facility staff prior to use of the histology area. Training is free and can be arranged with facility staff upon request. Competent users (as designated by facility technicians) will require area induction and sign off only.