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The Nikon A1R comprises a dual scanhead design, allowing the use of both the galvano and resonant scanners - offering the choice of extreme imaging speed, or high resolution.
With the Okolab environmental chamber and stage-top incubator, time lapse imaging of live samples is possible via temperature and air-mix control.
Extremely sensitive GaAsp detectors allow the imaging of the faintest signals and result in clear high-resolution results.
Acquisition and analysis are carried out from the Nikon NIS-Elements C software, which takes you from image acquisition to paper-ready figures in a single session.
A modern upright research microscope, the Ni-E is fitted with the Nikon DS-Fi3 camera for colour and emulated monochrome, allowing for high resolution brightfield and fluorescent imaging.
Controlled by the Nikon NIS-Elements software, the Ni-E is fast and easy to use. With 5 fluorescent channels, the system is extremely versatile.
The Axiovert 200M chassis has been fitted with a CoolLED PE300-Ultra LED light source for fast and reliable fluorescent imaging. The Retiga R6 EM-CCD camera provides high speed and high sensitivity, and the automated stage and environmental control chamber facilitates reliable live cell imaging.
Controlled via MicroManager software this platform is very flexible with all the advantages of a modern inverted widefield system.
The T-SPIM is a custom-built light sheet microscope featuring dual-sided illumination for even excitation of large samples such as zebrafish embryos and spheroids. Imaging is done in a sealed chamber wherein the sample is immersed in a selected medium (eg PBS or cell culture medium).
SPIM (Selective Plane Illumination Microscopy) combines the optical sectioning of a confocal microscope with the speed and sensitivity of widefield imaging by exciting the sample with a sheet of laser light, selectively illuminating only the imaging plane to minimize photobleaching and offsite excitation that impedes image resolution in conventional widefield microscopy.
The T-SPIM can be set up with either a 10x, 20x, or 40x detection objective depending on the sample to be imaged; please discuss your imaging needs with IRF staff in advance of your imaging session.
The NanoZoomer 2.0-RS slide scanner from Hamamatsu allows the scanning of up to six standard (76mm x 26mm) or two double-size (76mm x 52mm) slides in a variety of modes: manual, semi-automatic, and automatic.
Scanning can be done at 20x (0.46 µm/pixel) and 40x (0.23 µm/pixel) magnification, with scan times as fast as 1 minute 40 seconds for a 20mm by 20mm area at 20x magnification, with 40x magnification increasing the scan time proportionally.
This system allows the generation of high-resolution images of the entire slide, allowing image analysis to be done when required by the user, without the risk of missing information during image acquisition, since the entire slide is recorded.
The free NDP.view2 software allows viewing, annotation, and export of scans. NDP.Serve allows the hosting of slides online for viewing via a web browser.
The LiveCyte2 utilises Quantitative Phase Imaging (QPI) to generate label-free high contrast images for automatic quantification of live cells. The proprietary QPI technology allows the gentle imaging of even sensitive cell lines that would normally be prohibitive of live-labelled imaging.
The user-friendly software allows real-time analysis of live time-series experiments, meaning users can set up assays and collect fully processed figure-ready data at the end of the run, along with viable cells that can be passaged or processed for further experimentation.
Phase Focus provide a suite of analysis assays, for tracking a range of metrics in a variety of combinations, with applications in Cell Proliferation and Growth, Wound Healing, Cell Motility, Oncology, Toxicology, Angiogenesis and Stem Cell differentiation.
The ImageXpress Pico is a high-throughput widefield microscope for image acquisition in microwell plates and slides. It offers environmental control, including temperature and humidity, to maintain optimal conditions for live cell imaging experiments.
The ImageXpress Pico is equipped with a 1.3-megapixel sCMOS camera that can capture high-resolution images with low noise and high sensitivity. The system supports a wide range of fluorescence dyes and can be used for various fluorescence imaging assays. The system combines advanced optics, live cell imaging capabilities, and user-friendly software to provide high-quality images and efficient data analysis.
The software includes a range of analysis tools for quantifying cell morphology, measuring fluorescence intensity, and performing advanced image processing tasks such as mitochondria assay, angiogenesis, cell and nuclei count etc. It also allows the creation of customisable analysis workflows to automate image analysis and data extraction.
The BioTek autoscratch is an automated wound making tool that create a uniform monolayer of wound in 24 and 96 wells microplate using a single click. The autoscratch is permanently housed inside a class II biological safety cabinet. The device ensures reproducible wound size for wound healing assay.