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An interdisciplinary project to review leadership teaching for St George’s Undergraduate students
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All new users of the Image Resource Facility must register via PPMS and undergo a compulsory training session or workshop by a member of the IRF staff to ensure the trainee reaches a basic level of competency before permission is given to individuals to use our on line booking system or perform sample processing techniques.
Light microscopy training is provided as a free of charge service whilst the trainee is charged for microscope usage only. Microscope training sessions are usually 1-2 hours depending on the complexity of microscope operation or until confidence is achieved.
Histology and electron microscopy sample preparation workshops are available to researchers and students. The duration of each workshop is dependent on the complexity of the technique and charged at £25.00 per workshop. Workshops must be booked in advance by contacting the Image Resource Facility .
When planning a grant application please use the following charges as a guide only. Principle Investigators and researchers are encouraged to discuss their specific resource requirements so that the IRF can offer flexible and creative solutions. Annual fees are available and tailored to the individual project allowing the user unlimited open access to IRF resources and equipment.
Charges will be applied to the sub project code supplied on the registration form. Charges are submitted on a monthly basis and histology. For long collaborations charges will be reviewed and submitted every 3 - 6 months
For equipment specifications and details please click on the microscope and equipment links.
Confocal Laser Scanning Microscope (Inverted)
Zeiss LM510 META
Bio-Rad Confocal Nikon Eclipse E800
Confocal Laser Scanning Microscope (Upright)
Zeiss Axiovert 135 – Time Lapse
Epi-fluorescent microscope (Inverted)
Zeiss Axiovert 200M
Zeiss Axioplan 2
Light & Epi-fluorescent microscope (Upright)
* Please contact the IRF for time-lapse charging with duration and imaging requirements
Hamamatsu NanoZoomer 2.0-RS
High speed, high definition slide scanning 20x and 40x magnification
AMT 16MP high resolution CCD camera for ultrastructural examination and imaging
£80.00 p/half day
The following charges are for pilot projects and one time research projects. For long term collaborations or research projects with 6 or more samples required for EM processing and imaging please contact Maria McGlynn to discuss flexible prices and options.
Fixation & Routine processing & embedding into resin block
Leica Ultracut E - Semi-thin sectioning
(inclusive of toluidine routine stain)
Leica Ultracut E - Ultra-thin sectioning
(inclusive of Uranyl acetate & lead citrate contrast post staining)
Fixation & processing & Immunogold labelling into resin block
Thermo Scientific HM525 NX
Leica TP1020 - Automated Tissue Processor & Leica HC Acradia Embedding Station - processing & embed to wax block
* Processing and embedding is discounted to £5.00 per block when booking the Leica RM2255 microtome for continued sectioning.
Leica RM2255 Microtome - Wax block sectioning
Heamatoxylin and Eosin (H&E) - routine staining
Dewax, stain & mount with coverslip
Gomori's trichrome special stain for connective tissue
Miller's Elastic staining special stain - connective tissues, elastic fibers
Periodic Acid Schiff (PAS) - glycogen
* Please contact the IRF for other special histology staining protocols not listed
Immunohistochemistry antibodies (Available on request)
Note: work performed by IRF technical staff is prioritized depending on the demands of the workload of the facility and personnel availability.
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