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Internal Users Registration

All new users of the Image Resource Facility must register via PPMS and undergo a compulsory training session or workshop by a member of the IRF staff to ensure the trainee reaches a basic level of competency before permission is given to individuals to use our on line booking system or perform sample processing techniques.

Training and Workshops

Light microscopy training is provided as a free of charge service whilst the trainee is charged for microscope usage only. Microscope training sessions are usually 1-2 hours depending on the complexity of microscope operation or until confidence is achieved.

Histology and electron microscopy sample preparation workshops are available to researchers and students. The duration of each workshop is dependent on the complexity of the technique and charged at £25.00 per workshop. Workshops must be booked in advance by contacting the Image Resource Facility .

Grant Applications and Open Access

When planning a grant application please use the following charges as a guide only. Principle Investigators and researchers are encouraged to discuss their specific resource requirements so that the IRF can offer flexible and creative solutions. Annual fees are available and tailored to the individual project allowing the user unlimited open access to IRF resources and equipment.

Billing

Charges will be applied to the sub project code supplied on the registration form. Charges are submitted on a monthly basis and histology. For long collaborations charges will be reviewed and submitted every 3 - 6 months

Prices

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Light Microscopy

For equipment specifications and details please click on the microscope and equipment links.

Light microscopy prices
 Nikon A1R

Confocal Laser Scanning Microscope (Inverted)

Time-Lapse imaging*

 £40.00 p/hour

Zeiss LM510 META

Confocal Laser Scanning Microscope (Inverted)

Time-Lapse imaging*

 £20.00 p/hour

Bio-Rad Confocal Nikon Eclipse E800

Confocal Laser Scanning Microscope (Upright)

 £20.00 p/hour

Zeiss Axiovert 135 – Time Lapse

Epi-fluorescent microscope (Inverted)

Time-Lapse imaging*

 £20.00 p/hour

Zeiss Axiovert 200M

Epi-fluorescent microscope (Inverted)

 £15.00 p/hour

Zeiss Axioplan 2

Light & Epi-fluorescent microscope (Upright)

 £12.00 p/hour


* Please contact the IRF for time-lapse charging with duration and imaging requirements

High Resolution Slide Scanning

Hamamatsu NanoZoomer 2.0-RS

High speed, high definition slide scanning                                         
20x and 40x magnification

£20.00 p/hour
 
Image Analysis
Image analysis prices
 Nikon NIS-Elements C training workshops  £25.00 p/hour
 Nikon NIS-Elements C software   £10.00 p/hour
Electron Microscopy
Hitachi H-7100

AMT 16MP high resolution CCD camera for ultrastructural examination and imaging

  • £25.00 p/hour

  • £80.00 p/half day

  • £150.00 p/day

TEM Sample Preparation

The following charges are for pilot projects and one time research projects. For long term collaborations or research projects with 6 or more samples required for EM processing and imaging please contact Maria McGlynn to discuss flexible prices and options.

Fixation & Routine processing & embedding into resin block

£50.00 p/run
(1 - 5 specimens)

Leica Ultracut E - Semi-thin sectioning

(inclusive of toluidine routine stain)

£10.00 p/hour

Leica Ultracut E - Ultra-thin sectioning

(inclusive of Uranyl acetate & lead citrate contrast post staining)

£25.00 p/block

Fixation & processing & Immunogold labelling into resin block

£65.00 p/run
(1 - 5 specimens)
Cryo-sectioning of frozen specimen

Thermo Scientific HM525 NX                                  

£12.00 p/hour

Leica CM1900

£12.00 p/hour
Histology Sample Preparation

Leica TP1020  - Automated Tissue Processor & Leica HC Acradia
Embedding Station - processing & embed to wax block

£10.00p/block*

* Processing and embedding is discounted to £5.00 per block when booking the Leica RM2255 microtome for continued sectioning.

Leica RM2255 Microtome - Wax block sectioning

(excluding slides)

£10.00 p/hour

Heamatoxylin and Eosin (H&E) - routine staining

Dewax, stain & mount with coverslip

£2.00 p/slide

Gomori's trichrome special stain for connective tissue

Dewax, stain & mount with coverslip

£2.50 p/slide

Miller's Elastic staining special stain - connective tissues, elastic fibers

Dewax, stain & mount with coverslip

£2.50 p/slide

Periodic Acid Schiff (PAS) - glycogen

Dewax, stain & mount with coverslip

£2.50 p/slide

* Please contact the IRF for other special histology staining protocols not listed

Immunohistochemistry

Immunohistochemistry protocol

£10.00 p/slide

Immunohistochemistry antibodies (Available on request)

£1.00 p/aliquot

Note: work performed by IRF technical staff is prioritized depending on the demands of the workload of the facility and personnel availability.

 

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