Internal Users Registration
All new users of the Image Resource Facility must register via PPMS and undergo a compulsory training session or workshop by a member of the IRF staff to ensure the trainee reaches a basic level of competency before permission is given to individuals to use our on line booking system or perform sample processing techniques.
Training and Workshops
Light microscopy training is provided as a free of charge service whilst the trainee is charged for microscope usage only. Microscope training sessions are usually 1-2 hours depending on the complexity of microscope operation or until confidence is achieved.
Histology and electron microscopy sample preparation workshops are available to researchers and students. The duration of each workshop is dependent on the complexity of the technique and charged at £25.00 per workshop. Workshops must be booked in advance by contacting the Image Resource Facility .
Grant Applications and Open Access
When planning a grant application please use the following charges as a guide only. Principle Investigators and researchers are encouraged to discuss their specific resource requirements so that the IRF can offer flexible and creative solutions. Annual fees are available and tailored to the individual project allowing the user unlimited open access to IRF resources and equipment.
Billing
Charges will be applied to the sub project code supplied on the registration form. Charges are submitted on a monthly basis and histology. For long collaborations charges will be reviewed and submitted every 3 - 6 months
Prices
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For equipment specifications and details please click on the microscope and equipment links.
* Please contact the IRF for time-lapse charging with duration and imaging requirements
High Resolution Slide Scanning
Image Analysis
Image analysis prices
Nikon NIS-Elements C training workshops |
£25.00 p/hour |
Nikon NIS-Elements C software |
£10.00 p/hour |
AMT 16MP high resolution CCD camera for ultrastructural examination and imaging
-
£25.00 p/hour
-
£80.00 p/half day
-
£150.00 p/day
TEM Sample Preparation
The following charges are for pilot projects and one time research projects. For long term collaborations or research projects with 6 or more samples required for EM processing and imaging please contact Maria McGlynn to discuss flexible prices and options.
Fixation & Routine processing & embedding into resin block
|
£50.00 p/run (1 - 5 specimens) |
Leica Ultracut E - Semi-thin sectioning
(inclusive of toluidine routine stain)
|
£10.00 p/hour |
Leica Ultracut E - Ultra-thin sectioning
(inclusive of Uranyl acetate & lead citrate contrast post staining)
|
£25.00 p/block |
Fixation & processing & Immunogold labelling into resin block
|
£65.00 p/run (1 - 5 specimens) |
Cryo-sectioning of frozen specimen
Thermo Scientific HM525 NX
|
£12.00 p/hour |
Leica CM1900
|
£12.00 p/hour |
Histology Sample Preparation
Leica TP1020 - Automated Tissue Processor & Leica HC Acradia Embedding Station - processing & embed to wax block
|
£10.00p/block* |
* Processing and embedding is discounted to £5.00 per block when booking the Leica RM2255 microtome for continued sectioning.
Leica RM2255 Microtome - Wax block sectioning
(excluding slides)
|
£10.00 p/hour |
Heamatoxylin and Eosin (H&E) - routine staining
Dewax, stain & mount with coverslip
|
£2.00 p/slide |
Gomori's trichrome special stain for connective tissue
Dewax, stain & mount with coverslip
|
£2.50 p/slide |
Miller's Elastic staining special stain - connective tissues, elastic fibers
Dewax, stain & mount with coverslip
|
£2.50 p/slide |
Periodic Acid Schiff (PAS) - glycogen
Dewax, stain & mount with coverslip
|
£2.50 p/slide |
* Please contact the IRF for other special histology staining protocols not listed
Immunohistochemistry
Immunohistochemistry protocol
|
£10.00 p/slide |
Immunohistochemistry antibodies (Available on request)
|
£1.00 p/aliquot |
Note: work performed by IRF technical staff is prioritized depending on the demands of the workload of the facility and personnel availability.