Dr Tristan McKay is a Reader in Stem Cell Biology.
His research focuses on:
Mechanism of iPS reprograming. The ability to genetically reprogram human somatic cells to pluripotent stem cells (iPSc) has created huge potential for the study and treatment of a myriad of debilitating diseases. Currently, the mode of genetic manipulation to affect iPSc reprograming is highly efficient and not clinically efficacious. My group study the underlying mechanisms of iPSc reprograming in order to develop protocols that are more efficient, safer and efficacious.
Patient-derived iPSc as a platform to study complex genetic diseases. My group is generating iPSc lines from patients with diverse genetic disorders. These iPSc lines can then be differentiated to the cell-types affected by the disease and represent a unique cellular platform to study disease biochemistry in conjunction with next generation genomic and transcriptomic sequencing.
Genetic reporters of differentiation and disease in vitro and in vivo. The process of cellular differentiation and disease induction are controlled by gene expression. My group are developing a range of tools and technologies to quantitate genetic changes at the cellular, tissue and whole body level. Either fluorescent or bioluminescent transgenes driven by gene promoters that are activated by the progression of differentiation or disease are used to temporally quantitate activity.
Dr Tristan McKay joined St George's in April 2013 as Senior Lecturer in Stem Cell Biology. Prior to his appointment he was a Senior Lecturer at the William Harvey Research Institute, Queen Mary University of London where he retains an Honorary Senior Lectureship.
He gained experience as a stem cell biologist as a post-doctoral researcher and group leader at the North West Embryonic Stem Cell Centre (NWESCC) and UK Centre for Tissue Engineering (UKCTE) at the University of Manchester. He also gained post-doctoral experience working on gene therapies for Cystic Fibrosis at the CF/Pulmonary Research & Treatment Center, University of North Carolina, USA.
In 2000 Dr McKay gained an MSc (Dist) in Medical Genetics from Imperial College where he went on to obtain a PhD in CF Gene Therapy. He was awarded his first degree in Cell & Molecular Biology at the University of Essex.
Hawkins, K.E., Sharp, T.V. and McKay. T.R. The role of hypoxia in stem cell potency and differentiation. Regenerative Medicine. (2013) Vol. 8 (6): p771-82
Guasti, L., Sze, C., McKay, T. Grose, R. King, P.J. FGF signaling through Fgfr2 isoform IIIb regulates adrenal cortex development. Mol Cell Endocrinology. (2013) Vol. 371 (1-2): p182-8.
Trivellin, G., Butz, H., Delhove, J., Igreja, S., Chahal, H. S., Zivkovic, V., McKay, T., Patocs, A., Grossman, A. B. & Korbonits, M. MicroRNA miR-107 is overexpressed in pituitary adenomas and inhibits the expression of aryl hydrocarbon receptor-interacting protein in vitro. Am J Physiol Endocrinol Metab. (2012) Vol. 303; (6) E708-19.
McKay, T.R., Camarasa, M.V., Bates, N., Aplin, J.A., Brison, D.A. and Kimber S.J. Generation of an immortalized human feeder cell line for the xenofree culture of hES/hiPS cell lines Stem Cell Research (2011) Vol. 7 (2) p154-62.
Waddington, S.N., Crossley, R., Howe, S., Buckley, S., Reynolds, L., K., H.-D., Gilham, D.E., Hawkins, R.E., and McKay, T.R. Gene delivery of latency ablated TGF-β3 regulates dermal wound re-epithelialsation in mice by a β-3 integrin-specific mechanism and reduces scar tissue formation. Molecular Therapy (2010) Vol. 18(12); p2104-11.
Rothwell, D., Crossley, R., Sheard, V., Gilham, D.E., Zhang, Y., Sharp, T.V., Hawkins, R.E., and McKay, T.R. Expression, Cleavage and Secretion of TGF-B3 From an FMDV 2A Bicistronic Retroviral Cassette Is Position-dependent. Human Gene Therapy (2010) Vol. 21(11), p1631-7.
Zhang, L., Button, B., Gabriel, S.E., Burkett, S., Yan, Y., Skiadopoulos M.H., Dang, Y.L., Vogel, L.N., McKay, T.R., Mengos, A., Boucher, R.C., Collins, P.L., Pickles, R.J. CFTR delivery to 25% of surface epithelial cells restores normal rates of mucus transport to Cystic Fibrosis airway epithelium PLoS Biology (2009) July 7 (7).
Buckley, S. M., Howe, S. J., Wong, S. P., Buning, H., McIntosh, J., Baker, A., Nathwani, A. C., Thrasher, A., Coutelle, C.,Waddington, S. and McKay, T. R. Luciferin detection after intra-nasal vector delivery is improved by intra-nasal rather than intra-peritoneal luciferin administration. Human Gene Therapy (2008) 19 (10) p1050-6.
McKay, T., Patel, M., Pickles, R.J., Johnson, L.G., and Olsen, J.C. 2006. Influenza M2 envelope protein augments avian influenza hemagglutinin pseudotyping of lentiviral vectors. Gene Ther 13:715-724.
Doctor Tristan McKay supervises the following students:
Dr Kate Hawkins – Mechanisms of iPSc reprogramming.
Juliette Delhove – In vivo bioimaging.
Matt Shepherd – Targeted differentiation of pluripotent stem cells to hepatocytes.
Lorna FitzPatrick - Targeted differentiation of pluripotent stem cells to neurons.
Doctor Tristan McKay's collaborators include:
Dr Simon Waddington (Institute for Women’s Health, University College London).
Dr Tyson Sharp (Bart’s Cancer Institute, Queen Margaret University London).
Professor Leo Dunkel (William Harvey Research Institute, Queen Margaret University London).
Professor Andrew Tinker (William Harvey Research Institute, Queen Margaret University London).
Dr Elijah Behr (St George’s, University of London).
Dr Pia Ostegaard (St George’s, University of London).
St George’s, University of London Enterprise Fund
Postgraduate – PhD students.