We are currently offering the following studentship.
Supervisors: Professor Tom Carter, Dr Katalin Torok
Molecular basis for regulated protein secretion from vascular endothelial cells
Our aim is to understand the molecular mechanisms controlling the intracellular trafficking and regulated secretion of proteins and peptides from vascular endothelial cells.
Endothelial cells are highly specialized secretory cells that line the lumen of all blood vessels, the network of tubes that carry blood to the tissues and organs of the body. Endothelial cells regulate vital processes within the vascular network, including blood flow, blood clotting, inflammation, vessel growth and repair. They do this through the secretion a host of different proteins with vasoactive, adhesive, anti-adhesive, coagulant, angiogenic, mitogenic and inflammatory functions. Endothelial cell damage or dysfunction alters the composition or balance of the secreted molecules, and this can predispose to an increased risk of vascular disease, infection and cancer.
Proteins destined for regulated secretion from endothelial cells are stored in special secretory granules called Weibel-Palade bodies (WPBs). This project will explore the role of identified candidate molecules in the control of hormone triggered WPB exocytosis and cargo secretion using a unique tool box of fluorescent reporters (developed in our lab) that allow the trafficking and exocytosis of WPBs to be directly visualised in living endothelial cells. The project will involve the application of biochemical, molecular and cell biological techniques, high speed multi-colour live cell fluorescence imaging and electrochemical approaches for monitoring secretory granule fusion, all of which are established in the lab.
We are seeking a highly motivated, outstanding individual with a first or 2:1 degree in the physical or biological sciences and preferably with a Masters degree or previous lab experience. The candidate should have a keen interest in learning and applying biophysical and molecular cell techniques and have exceptional analytical skills. You will receive training in primary cell culture cell, biochemical and molecular techniques, fluorescence (including wide field, TIRF and confocal) imaging, image analysis, whole cell patch clamp and amperometry, data analysis, data presentation and paper writing.
The application form must be submitted by: 5pm, 5 April 2013.
Please complete the reference form and return by 11 April 2014.
Application form 2014 (Word)
Reference request form (Word)
The project is funded for 3 years and successful applicants will be awarded a non-taxable annual stipend starting at £15,988, plus payment of university tuition fees. For enquiries please contact:
Tel: 020 88162743